Method and apparatus for transferring growth media and infection fluids to a cell growth bag
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United States of America Patent
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Aug 16, 2011
Grant Date -
Sep 2, 2010
app pub date -
Apr 30, 2010
filing date -
Dec 2, 2005
priority date (Note) -
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status (Latency Note)
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Abstract
A method and apparatus are provided for transferring growth media or infection fluids to a bioculture bag having Could Process fitting thereon in fluid communication with the inside of the bag. The media or fluid is placed in a flask. A cap is threaded onto the neck of the flask. The cap has a spout with an opening in a distal end of the spout and a vent hole in the cap. The spout has a tubular end in which the opening is formed, with an O-ring seal adjacent the distal end of the tubular end. The tubular end fits within the fitting and the seal forms a fluid tight seal with the fitting to allow fluid transfer to the bag in reduced time with reduced spillage. The cap is preferably used on a flask having shaped baffles in the bottom. The baffles are highest toward the centerline, and decline in height linearly toward the corners of the flask at an angle of about 9° to the horizontal. The flask is rotated about 80-180 RPM, and the baffles have leading and trailing sides in the direction of rotation that are inclined at about 32° relative to the vertical. A growth media is also provided to culture the cells in the flask.
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