Method of preparing basement membrane, method of constructing basement membrane specimen, reconstituted artificial tissue using the basement membrane specimen and process for producing the same
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United States of America Patent
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Jul 5, 2011
Grant Date -
Mar 22, 2007
app pub date -
Nov 15, 2006
filing date -
Sep 25, 2001
priority date (Note) -
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Abstract
A reconstructed artificial tissue is obtained by seeding and culturing desired homogeneous or heterogeneous cells having an ability to form a basement membrane on the basement membrane specimen constructed by the following process: the cells having an ability to form a basement membrane adhered onto a support structure through a basement membrane are treated with a surface active agent; the lipid component of cells is lysed; the mixture of an alkaline solution and a protease inhibitor is used to lyse the protein remained on the surface of the basement membrane of the cells. A protein support structure is temporarily adhered to plastic surface through an adsorptive polymer by hydrophobic bonding, such as an alternating copolymer of methyl vinyl ether and maleic anhydride, which has a hydrophobic linear carbon skeleton and a functional group which can react with protein in a molecule and a basement membrane specimen or an artificial tissue is formed thereon.
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