Fluorescent primer system for detection of nucleic acids (Q priming)
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United States of America Patent
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Jun 21, 2011
Grant Date -
Sep 3, 2009
app pub date -
Dec 5, 2006
filing date -
Dec 5, 2005
priority date (Note) -
Expired
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Abstract
The present invention is directed to a self-quenching primer comprising; a fluorophore that can be quenched by guanine; an oligonucleotide sequence that forms a hairpin; an oligonucleotide that is a target specific sequence and; use in amplification reactions, particularly in polymerase chain reactions, during which the fluorophore is released thereby emitting fluorescence.
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