Method and device for determining the fluorescence of a sample and use thereof
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United States of America Patent
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May 17, 2011
Grant Date -
Nov 19, 2009
app pub date -
Dec 15, 2006
filing date -
Dec 16, 2005
priority date (Note) -
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Abstract
In a method and a device for determining the fluorescence of a sample, wherein the sample (40) is irradiated by light (25) of a wavelength which is suitable to excite fluorescent light (27, 28) in the sample and the fluorescent light (28) emitted by the sample is received in a receiver (24) and converted into a measurement signal, wherein reference light (32) in particular for compensating for ambient influences is additionally fed to the receiver (24) and likewise converted into a reference measurement signal, provision is made for the optical path of the excitation light (25) entering the sample (40) and fluorescent light (27, 28) leaving the sample (40) to be separated from the optical path (32) of the reference light having the same wavelength as the excitation light between light sources (21, 31) and receiver (24), by means of which a more precise evaluation of the fluorescent light emitted by a sample (40) can be achieved in addition to a simplified design complexity.
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