One step oligochromatographic device and method of use
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United States of America Patent
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Apr 26, 2011
Grant Date -
Aug 30, 2007
app pub date -
Apr 30, 2004
filing date -
May 7, 2003
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Abstract
The present invention relates to methods and devices for detecting one or more analytes in a biological sample, preferably a clean liquid sample. This invention in particular relates to improved rapid tests such as “dipsticks”, “lateral flow” devices and “flow-through” devices. The invention in particular relates to oligochromatographic devices that make use of a peptide- or hapten-coupled oligonucleotide and a reagent specifically recognizing the hapten or peptide and a conjugated probe that hybridizes specifically to a target sequence. It allows to detect specifically the presence of a polynucleotides directly or after molecular amplification steps with the use of a specific genuine internal control and a chromatographic control.
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