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Abstract

Disclosed is a novel method for determination of ProGRP which is free from problems such as the dispersion in the values of measurements and operational constraints such as handling of a test sample. A method for the determination of a gastrin-releasing peptide precursor or a digest thereof using at least two different antibodies each of which can recognize a peptide comprising the partial amino acid sequence starting from amino acid residue 40 and ended at amino acid residue 75 in the amino acid sequence depicted in SEQ ID NO:1; and a method for the determination of a gastrin-releasing peptide precursor and/or a digest thereof using at least two different antibodies each of which can recognize a peptide comprising the partial amino acid sequence starting from amino acid residue 40 and ended at amino acid residue 79 in the amino acid sequence depicted in SEQ ID NO:1. The methods have such advantages that a detection sensitivity comparable to that in a conventional determination method can be achieved, that it is easy to handle a sample after the sample is collected, and that a highly reproducible measurements can be obtained.

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