Fluorescence-based scanning imaging device
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United States of America Patent
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Mar 22, 2011
Grant Date -
Sep 3, 2009
app pub date -
Nov 7, 2006
filing date -
Nov 9, 2005
priority date (Note) -
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Abstract
A device for analysing a specimen by fluorescence includes a confocal microscope, illumination means capable of emitting a light beam that converges, by means of an objective, on a focal spot, means for successively positioning the focal spot at various points on the specimen during analysis. The confocal microscope includes an objective mounted on a movable rapid-scan carriage driven in a reciprocating linear movement along a traverse direction by a rotating motor by means of a device of the connecting rod type. The specimen is placed on a movable support driven in a longitudinal movement and is able to move along the axis of the objective of the microscope in order to position the specimen relative to the focal spot. The excitation light spectrum is spread over the surface of the specimen in such a way that the excitation light reflected by the specimen and corresponding to the wavelengths close to fluorescence converge on points that are sufficiently distant from a diaphragm positioned in front of a device for measuring the fluorescence.
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