Single enzyme system for fast, ultra long PCR

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United States of America Patent

PATENT NO 7892797
APP PUB NO 20090170167A1
SERIAL NO

12115198

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Abstract

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The present invention provides methods, formulation and kits for the synthesis of long nucleic acid fragments. An improved PCR method is provided for amplifying long DNA fragments. In particular, a single thermostable DNA polymerase is used for the rapid amplification of over 10 kb long DNA fragments. Also provided is a method for extending long complementary DNA strands using this single enzyme formulation.

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Patent Owner(s)

Patent OwnerAddress
GE HEALTHCARE BIO-SCIENCES CORPPISCATAWAY NJ 08855

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kumar, Gyanendra Franklin Park, US 16 266
Mitsis, Paul Trenton, US 6 412
Sekher, Anuradha Belle Mead, US 10 99

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