Method of performing PCR amplification on a microarray
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United States of America Patent
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Feb 22, 2011
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Oct 13, 2005
app pub date -
Jan 12, 2005
filing date -
Jan 12, 2004
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Abstract
The present invention provides a method of amplifying target DNA by PCR or multiplex PCR on a microarray using array-immobilized DNA probes synthesized in a common area on the microarray by a maskless array synthesizer (MAS). The MAS constructed array-immobilized DNA probes include a universal primer linked to a sequence-specific probe, and optionally a calibrated probe for use in quantifying amplified target DNA.
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