3D biplane microscopy
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Feb 1, 2011
Issued Date -
N/A
app pub date -
Jun 29, 2010
filing date -
Apr 1, 2008
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
A microscopy system is configured for creating 3D images from individually localized probe molecules. The microscopy system includes a sample stage, an activation light source, a readout light source, a beam splitting device, at least one camera, and a controller. The activation light source activates probes of at least one probe subset of photo-sensitive luminescent probes, and the readout light source causes luminescence light from the activated probes. The beam splitting device splits the luminescence light into at least two paths to create at least two detection planes that correspond to the same or different number of object planes of the sample. The camera detects simultaneously the at least two detection planes, the number of object planes being represented in the camera by the same number of recorded regions of interest. The controller is programmable to combine a signal from the regions of interest into a 3D data.
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