Electrochemical method to measure DNA attachment to an electrode surface in the presence of molecular oxygen
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United States of America Patent
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Feb 1, 2011
Grant Date -
Jan 28, 2010
app pub date -
Sep 29, 2009
filing date -
Nov 6, 2002
priority date (Note) -
Expired
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Abstract
The present disclosure provides methods and compositions for conducting an assay to detect a polynucleotide. In particular, ruthenium complexes having reduction potentials that do not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the polynucleotide that is detected is DNA. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.
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