Process for the removal of exocyclic base protecting groups
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United States of America Patent
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Jan 18, 2011
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app pub date -
Apr 15, 2005
filing date -
Apr 19, 2004
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Abstract
Nitrogen-protecting groups are removed from the exocyclic nucleobase portion of a 2′-O protected nucleotide or 2′-halo nucleotide by contacting the nucleotide with an inorganic base. Typical is the removal of t-butylphenoxyacetyl protecting groups from the nucleobase portion of a 2′-O protected nucleotide on which the 2′-O protecting group is t-butyldimethylsilyl, removal or deprotection being accomplished by contact with a potassium carbonate solution.
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