Enhanced cell-free synthesis of active proteins containing disulfide bonds
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United States of America Patent
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Jan 18, 2011
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Jan 18, 2008
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Jan 18, 2007
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Abstract
Compositions and methods are provided for the enhanced in vitro synthesis of active polypeptides containing disulfide bonds. In certain embodiments of the invention, the reaction mix includes a biological extract derived from a bacterial cell in which the glutathione reductase gene has been inactivated, which is pre-treated with a low concentration of a sulfhydryl inactivating agent.
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