Search for cancer markers by a novel screening method
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United States of America Patent
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Jan 11, 2011
Grant Date -
Jan 24, 2008
app pub date -
Feb 9, 2007
filing date -
Sep 25, 2001
priority date (Note) -
Expired
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Abstract
The present invention relates to a method of concentrating low molecular weight peptides in the supernatant of serum-free cultured cells, said method comprising allowing the peptides to bind to a strong cation exchanger under an acid condition, and eluting them under an alkali condition to concentrate the peptide. Furthermore, peptides having the amino acid sequence as set forth in SEQ ID NO: 1 or 2, and a method of screening cancer markers using antibody to these peptides, are disclosed.
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