DNA polymerases with enhanced length of primer extension
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United States of America Patent
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Dec 21, 2010
Grant Date -
Jun 11, 2009
app pub date -
Oct 21, 2008
filing date -
Feb 19, 1993
priority date (Note) -
Expired
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Abstract
A formulation and kit of thermostable or other DNA polymerases comprising at least one thermostable or other DNA polymerase which lacks 3′-exonuclease activity, and at least one thermostable DNA polymerase exhibiting 3′-exonuclease activity. Also provided is an improved method for enzymatic extension of DNA strands, especially while, but not limited to, amplifying nucleic acid sequences by polymerase chain reaction wherein the above formulation is made and used to catalyze primer extension.
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