Bidimensional separation by means of capillary electrophoresis carried out in a single capillary
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United States of America Patent
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Nov 23, 2010
Grant Date -
Nov 16, 2006
app pub date -
Aug 23, 2004
filing date -
Aug 29, 2003
priority date (Note) -
Expired
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Abstract
A method for separating the constituents of a mixture M by electrophoresis in a single capillary includes (A) separating compounds of the mixture M in a single capillary according to the capillary electrophoresis technique; (B) isolating a fraction F of the compounds thus separated by evacuating part of the compounds having the highest migration speeds from the capillary and/or evacuating part of the compounds having the lowest migration speeds from the capillary; (C) introducing a separating medium MS having a higher migration speed than the compounds of the isolated fraction F into the capillary containing the isolated fraction F, and (D) the compounds contained in fraction F are separated in the new electrophoretic conditions thus obtained.
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