Methods for producing Factor VIII proteins
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United States of America Patent
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Oct 26, 2010
Grant Date -
Jul 28, 2005
app pub date -
Jan 26, 2005
filing date -
Mar 12, 1998
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Abstract
Methods are provided for purification of Factor VIII polypeptides by affinity chromatography and ion exchange chromatography, in which the eluate from the affinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The affinity matrix may comprise a monoclonal antibody or a peptide ligand. The methods result in improved purification without significant yield loss.
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