Degenerate oligonucleotide gene shuffling
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United States of America Patent
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Aug 10, 2010
Grant Date -
Mar 18, 2004
app pub date -
Aug 29, 2001
filing date -
Aug 29, 2000
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Abstract
A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5′ and 3′ degenerate ends.
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