Nucleic acid probes and microarrays for analysis of polynucleotides
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United States of America Patent
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Jul 13, 2010
Grant Date -
Jul 26, 2007
app pub date -
Oct 31, 2006
filing date -
Jan 25, 2006
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Abstract
The invention provides a probe for detecting a target polynucleotide. The probe contains a region that base-pairs with a target polynucleotide to form a duplex and a RNA hairpin extension domain that increases the stability of the duplex. The probe may further include a nucleotide clamp, a stem-complementary region and/or a linker moiety. Also provided is an array of subject probes bound to a surface of a solid support. Methods of using a subject probe to assess target polynucleotides, e.g., small RNAs, in a sample are provided, as are kits for use in practicing the subject methods.
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