Method of amplifying ATP and use thereof

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United States of America Patent

PATENT NO 7745160
APP PUB NO 20080213754A1
SERIAL NO

10566957

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Abstract

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The ATP amplification method of the present invention is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. The present invention also provides an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.

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Patent Owner(s)

Patent OwnerAddress
CALPIS CO LTD20-3 EBISU-NISHI 2-CHOME SHIBUYA-KU TOKYO 150-0021

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kuroda, Akio Hiroshima, JP 63 323

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