DNA cloning method

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United States of America Patent

PATENT NO 7736851
APP PUB NO 20040203057A1
SERIAL NO

10842534

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Abstract

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The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.

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Patent Owner(s)

Patent OwnerAddress
EUROPAISCHES LABORATORIUM FUR MOLEKULARBIOLOGIE (EMBL)D-69117 HEIDELBERG

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Buchholz, Frank Bremen, DE 26 194
Stewart, Francis Leimen, DE 10 137
Zhang, Youming Heidelberg, DE 34 177

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