Method for removing enzyme and method of base exchange or hydrolysis of phospholipid using the same
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United States of America Patent
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May 4, 2010
Grant Date -
Oct 9, 2008
app pub date -
Mar 18, 2005
filing date -
Mar 18, 2004
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Abstract
A method of removing an enzyme from a liquid enzyme reaction mixture used in a hydrolysis reaction or a base exchange reaction of a phospholipid is provided. The method includes the step of treating the liquid enzyme reaction mixture with a solvent mixture of water and an organic solvent, wherein the solvent mixture includes an inorganic metal salt, to remove the enzyme. Enzymes included in the reaction product can be easily removed without a treatment such as heating, and thus it becomes possible to easily produce various phospholipids that have a reduced risk of inducing an allergy, that retain a high quality and that have excellent storage stability.
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