Method for the detection of phosphine in cereals
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United States of America Patent
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Mar 16, 2010
Grant Date -
Jan 31, 2008
app pub date -
Jul 27, 2007
filing date -
Jul 28, 2006
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A method is described for the total phosphine detection in cereal caryopses, comprising the steps of inserting a sample of cereal caryopses inside a container (1) equipped with hermetic closure (2); adding to the sample an aqueous solution of H.sub.2SO.sub.4 with a v/v concentration in the range of 5-20%, with obtainment of an aqueous dispersion, and hermetically closing the first container (1); subjecting the aqueous dispersion contained in the first container (1) to the action of microwaves for a time not greater than 3 minutes; drawing a predetermined volume of gas overlying the aqueous dispersion and detecting the phosphine possibly present by means of colorimetric and/or spectrophotometric methods, preferably by bringing it into contact with a predetermined volume of an aqueous solution of AgNO.sub.3 of known molarity, inside a second container (3) with hermetic closure and visually analysing the obtained color and/or spectrophotometrically measuring the absorbance at 400 nm of the aqueous solution. Equipment for the execution of the aforesaid method is described.
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