Determination of light absorption pathlength in a vertical-beam photometer
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United States of America Patent
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Feb 16, 2010
Grant Date -
Nov 20, 2008
app pub date -
Feb 25, 2008
filing date -
Dec 23, 1998
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Abstract
Photometric methods for determining optical pathlength of liquid samples containing analytes dissolved or suspended in a solvent are provided that rely on determining a relationship between the light absorption properties of the solvent and the optical pathlength of liquid samples containing the solvent. This relationship is used to establish the optical pathlength for samples containing an unknown concentration of analyte but having similar solvent composition. Methods are also provided for determining the concentration of analyte in such samples where both the optical pathlength and the concentration of analyte are unknown. The methods may rely on separately determining, at different wavelengths of light, light absorption by the solvent and light absorption by the analyte. Light absorption by the analyte, together with the optical pathlength so determined, is used to calculate the concentration of the analyte.
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