Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
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Nov 24, 2009
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Oct 27, 2006
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Jun 26, 2002
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Abstract
A method for purifying bone-derived osteoinductive proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recovery process. The high and low ultrafiltration processes preferably select proteins having a nominal molecular weight between approximately 8 kilodaltons and approximately 100 kilodaltons. Processes of the present invention may be used to recover osteoinductive proteins from bone demineralization waste streams.
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