Amplicon melting analysis with saturation dyes
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United States of America Patent
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Sep 1, 2009
Grant Date -
Jan 26, 2006
app pub date -
Oct 22, 2003
filing date -
Oct 23, 2002
priority date (Note) -
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Abstract
Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
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