Expression vector encoding a triterpene hydroxylase polypeptide
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United States of America Patent
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Aug 18, 2009
Grant Date -
Jan 3, 2008
app pub date -
Feb 25, 2005
filing date -
Feb 25, 2004
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Abstract
Soyasapogenol B is biosynthesized via two steps of hydroxylation reaction of its precursor β-amyrin. However, the gene of the hydroxylase concerned in this reaction has not been revealed. Therefore, it was impossible to apply a genetic engineering technique on the hydroxylase. The present inventors reveals that a sequence which corresponds to a soybean-derived cytochrome P-450 gene CYP93E1 encodes an enzyme protein that carries out hydroxylation of the 24-position of an oleanane type triterpene, and also provides a method for applying said gene making use of a genetic engineering technique.
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