Microscope with a viewing direction perpendicular to the illumination direction

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United States of America Patent

PATENT NO 7554725
SERIAL NO

10538081

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Abstract

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The invention relates to a microscope, in which a layer of the sample is illuminated by a thin strip of light (11) and the sample is viewed (5) perpendicular to the plane of the strip of light. The depth of the strip of light (11) thus essentially determines the depth of focus of the system. To record the image, the object (4) is displaced through the strip of light (11), which remains fixed in relation to the detector (8), and fluorescent and/or diffused light is captured by a planar detector. Objects (4) that absorb or diffuse a large amount of light are viewed from several spatial directions. The three-dimensional images, which are captured from each direction can be combined retrospectively to form one image, in which the data is weighted according to its resolution. The resolution of the combined image is then dominated by the lateral resolution of the individual images.

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Patent Owner(s)

Patent OwnerAddress
EUROPAEISCHES LABORATORIUM FUER MOLEKULARBIOLOGIER (EMBL)MEYERHOFSTR 1 69117 HEIDELBERG

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Enders, Sebastian Heidelberg, DE 4 162
Huisken, Jan Heidelberg, DE 8 149
Lindek, Steffen Plankstadt, DE 7 159
Stelzer, Ernst H K Meckesheim, DE 12 182
Swoger, James H Heidelberg, DE 3 89

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