Method for measurement of activity of homocysteinethiolactone hydrolyzing enzyme
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United States of America Patent
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May 19, 2009
Grant Date -
Oct 18, 2007
app pub date -
Apr 12, 2005
filing date -
Apr 19, 2004
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Abstract
The present invention provides a method for the measurement of the activity of homocysteinethiolactone hydrolyzing enzyme in a sample. The method involves mixing and reacting γ-thiobutyrolactone and the sample in the presence of a cholinesterase inhibitor, and measuring 4-mercaptobutyric acid product. The method is safe and easy to operate, since it does not involve the use of a radioactive substrate, and is free of influence of other compounds contained in a bodily sample.
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