Liquid-gas-phase exposure reactor for cell culturing
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United States of America Patent
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May 12, 2009
Grant Date -
Jan 26, 2006
app pub date -
Jun 14, 2005
filing date -
Jun 14, 2004
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Abstract
Initiation of growth and cultivation of cells can be performed by introducing the cells into culture compartments of a liquid-gas-phase exposure bioreactor containing a supply chamber in which there are disposed hollow-filament membranes having an inside diameter of no larger than 5 mm, wherein an inner volume of said hollow-filament membranes forms the culture compartments. Approximately one half of the supply chamber is filled with a nutrient medium and a remainder is filled with a gas mixture. Perfusion of medium and gas is turned on simultaneously or separately. The hollow-filament membranes and the cells contained therein are cyclically exposed to the gas or liquid phase.
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