Method and apparatus for amplification of nucleic acid sequences using immobilized DNA polymerase

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United States of America Patent

PATENT NO 7488595
APP PUB NO 20040191830A1
SERIAL NO

10836376

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present invention generally relates to methods and apparatuses for amplifying nucleic acid sequences using immobilized DNA polymerase. More particularly, it relates to methods and apparatuses useful for amplifying target nucleic acid sequences by forming a plurality of reaction regions in which polymerase chain reaction (PCR) can occur, positioning immobilized DNA polymerase in a specific reaction region, and circulating DNA through the reaction regions. The present invention provides those methods and apparatuses that allow simple separation and recovery of the DNA polymerase after the amplification, that can be operated not only with thermostable DNA polymerases but also with non-thermostable DNA polymerases, and that are simpler in their designs and processes so that they can be readily integrated into complex devices such as Lab-on-a-chip.

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Patent Owner(s)

Patent OwnerAddress
AHRAM BIOSYSTEMS INCSEOUL 133-833

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hwang, Hyun Jin Seoul, KR 16 125
Jeong, Kyunghoon Gwangju, KR 6 68
Kim, Jeong Hee Seoul, KR 16 152

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