Affinity trap reactor and single-step process for purifying angiostatin-like fragment from human plasma using the same
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United States of America Patent
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Jan 13, 2009
Grant Date -
Jun 22, 2006
app pub date -
Jan 17, 2003
filing date -
Jan 17, 2003
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Abstract
The present invention provides an affinity trap reactor that enables the reaction between an enzyme bound to a support and substrate to proceed efficiently without its applications being restricted by the type of enzyme and substrate used. The present invention relates to an affinity trap reactor composed of a support bound with an enzyme and a molecule that specifically binds with a substrate of the enzyme, and a single-stage process for obtaining BL-angiostatin from plasminogen contained in a biological sample, wherein a biological sample containing plasminogen is applied to an affinity trap reactor composed of a support bound with bacillolysin MA and lysine, and reacted under conditions of a temperature of 0 to 50° C. in the presence of isopropyl alcohol but in the absence of calcium ions.
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