Denaturant-free electrophoresis of biological molecules under high temperature conditions
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United States of America Patent
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Jan 6, 2009
Grant Date -
Dec 23, 2004
app pub date -
Sep 15, 2003
filing date -
Apr 25, 2000
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Abstract
The present invention relates to a method of separating a sample comprising biological compounds, such as nucleic acids. The nucleic acids are subjected to electrophoresis using a matrix that is essentially free of denaturants and having at least one random, linear copolymer comprising a first comonomer of acrylamide and at least one secondary comonomer. A temperature of at least a portion of the matrix is at least about 80° C.
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