Method for detecting whether an organism is homozygous or heterozygous using labelled primers and RFLP
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United States of America Patent
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Dec 2, 2008
Grant Date -
Jul 8, 2004
app pub date -
Dec 20, 2001
filing date -
Dec 20, 2000
priority date (Note) -
Expired
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Abstract
The invention is a method for detecting whether an organism is homozygous or heterozygous in a target nucleotide. The method combines hybridization of two differently labelled probes A and B on either side of a restriction site, restriction endonuclease digestion, and detection of the labels in the resulting digest. If an organism is: homozygous then the labels will be present in the ratio 1:1 or 1:0; or if heterozygous then the labels will be present in the ratio 1:2. Either immobilized array technology or electrophoretic separation may be used.
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