Immunological detection of RNA:DNA hybrids on microarrays
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United States of America Patent
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Jul 15, 2008
Grant Date -
Sep 16, 2004
app pub date -
Dec 11, 2003
filing date -
Feb 6, 1998
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Abstract
The present invention provides a hybridization method and kit for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or optionally a detectably-labeled complementary biomolecule to form a double-stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification. Therefore, the present invention provides a method and kit to detect and measure biological molecules that is simple to use, highly specific, sensitive, and accurate for screening a plurality of biological molecules.
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