Fiber-optic assay apparatus based on phase-shift interferometry
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United States of America Patent
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Jul 1, 2008
Grant Date -
Nov 17, 2005
app pub date -
Nov 4, 2004
filing date -
Nov 6, 2003
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Abstract
Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance 'd' greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.
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