Detection and identification of staphylococcus aureus and epidermidis using the 16S-23S rRNA spacer
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United States of America Patent
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Jun 24, 2008
Grant Date -
Jun 30, 2005
app pub date -
Jul 21, 2004
filing date -
Jun 24, 1994
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Expired
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Abstract
The present invention relates to 16S-23S rRNA spacer sequences from Staphylococcus aureus and their use in a method for detection and/or identification of Staphylococcus aureus . The invention further relates to a method for detection and identification of Staphylococcus aureus in a sample, involving the steps of: (i) optionally releasing, isolating and/or concentrating the polynucleic acids present in the sample; (ii) optionally amplifying the 16S-23S rRNA spacer region, or a part thereof, with at least one primer pair; (iii) detecting the presence of a 16S-23S rRNA spacer sequence; and (iv) identifying the Staphylococcus aureus present in the sample from the nucleic acid(s) detected in the sample.
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