Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
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United States of America Patent
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Mar 4, 2008
Grant Date -
Oct 2, 2003
app pub date -
May 3, 2002
filing date -
May 4, 2001
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Abstract
The present invention relates to a temperature-stable labeling reagent of formula: ##STR00001## in which R.sup.1 represents H or an alkyl, aryl or substituted aryl group, R.sup.2 represents a detectable marker or at least two detectable markers linked together by at least one multimeric structure, L is a linking arm containing a linear succession of at least two covalent bonds and n an integer equal to 0 or 1, R.sup.3 and R.sup.4 represent independently of each other: H, NO.sub.2, Cl, Br, F, I, R.sup.2-(L).sub.n-Y--X--, OR, SR, NR.sub.2, R, NHCOR, CONHR, COOR with R=alkyl or aryl, A is a linking arm containing at least one covalent double bond allowing conjugation of the diazo functional group with the aromatic ring and u is an integer between 0 and 2, preferably equal to 0 or 1, and --Y--X-- represents --CONH--, --NHCO--, --CH.sub.2O--, --CH.sub.2S--. The present invention also describes a method for synthesizing said markers as well as applications for labeling biological molecules, in particular nucleic acids, with a labeling reagent carrying the diazomethyl functional group.The invention finds a preferred application in the field of diagnosis.
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