Plasmid shuttle vector between Escherichia coli and Brevibacillus
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Feb 19, 2008
Grant Date -
Apr 15, 2004
app pub date -
Feb 7, 2002
filing date -
Feb 14, 2001
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
The invention provides a plasmid shuttle vector characterized by being replicable in both of bacteria of the genus Brevibacillus and Escherichia coli, and by comprising a promoter capable of functioning in bacteria of the genus Brevibacillus and a DNA sequence(s) capable of functioning as a selective marker(s) for Escherichia coli and bacteria of the genus Brevibacillus, and a method of transforming bacteria of the genus Brevibacillus using the said plasmid shuttle vector. The plasmid shuttle vector enables the efficient transformation of bacteria of the genus Brevibacillus. Further, since plasmid shuttle vector pNCMO2 of the invention in particular has a gene expression control region which efficiently expresses and secretes an objective gene in bacteria of the genus Brevibacillus but significantly suppresses the expression in Escherichia coli, a recombinant gene expression plasmid can efficiently be constructed using Escherichia coli, and it is suited for protein production in bacteria of the genus Brevibacillus.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully