Screening assay for enzymes involved in pathophysiology using SELDI-TOF mass spectrometry
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United States of America Patent
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Feb 19, 2008
Grant Date -
Jul 20, 2006
app pub date -
Dec 2, 2005
filing date -
Dec 2, 2004
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Expired
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Abstract
The invention provides methods for quantifying enzymatic activity of an enzyme with a known substrate. The methods employ SELDI-TOF mass spectrometry, and are suitable, in particular, for assaying aspects of the renin-angiotensin system. The methods may be utilized to assess and/or monitor biological conditions associated with the renin-angiotensin system prior to the manifestation of known physiological and biomarkers for such conditions. The methods are suitable for analysis of pharmacological effectors of the renin-angiotensin system, and are particularly suitable for automation and high-throughput screening assay design.
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