Methods and materials for optimization of electronic transportation and hybridization reactions
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United States of America Patent
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Jan 1, 2008
Grant Date -
Mar 30, 2006
app pub date -
Sep 22, 2005
filing date -
Sep 23, 2004
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Abstract
Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.
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