Electroporation device and method, delivering a modulated signal under continuous control of cell electropermeabilization
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United States of America Patent
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Dec 11, 2007
Grant Date -
Feb 26, 2004
app pub date -
Oct 21, 2002
filing date -
Apr 21, 2000
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Abstract
An electroporation device having a signal generating circuit (3) connectable at the output to electrodes (5) fittable to a substrate (35) having cells. The electrodes (5) produce an electric field which induces permeabilization of the cell membranes to facilitate introduction of substances into the cells. The amplitude of the signal (12) applied to the electrodes is closed-loop controlled on the basis of the impedance (Z(.omega.)) of the substrate, so as to regulate the amplitude of the signal following a reduction in impedance caused by permeabilization of the cell membranes.
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