Method for setting a fluorescence spectrum measurement system for microscopy
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United States of America Patent
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Oct 9, 2007
Grant Date -
Mar 3, 2005
app pub date -
Aug 24, 2004
filing date -
Aug 27, 2003
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Abstract
A method for separating fluorescence spectra of dyes present in a specimen (15) is disclosed. Firstly a spectral scan of the fluorescence spectrum of all the dyes present in the specimen (15) is performed. The fluorescence spectra associated with the respective dyes are stored in a database of the computer system. After spectral deconvolution of the acquired mixed fluorescence spectrum, a comparison is made between the measured individual spectra ascertained by spectral deconvolution and the fluorescence spectra associated with the respective dyes. Lastly, a linear deconvolution of the acquired mixed fluorescence spectrum is performed.
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