Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof
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United States of America Patent
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Oct 9, 2007
Grant Date -
Aug 26, 2004
app pub date -
Apr 2, 2004
filing date -
Jun 4, 1998
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Abstract
A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.
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