Protein and process for preparing the same
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United States of America Patent
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Sep 11, 2007
Grant Date -
Sep 25, 2003
app pub date -
Feb 12, 2003
filing date -
Apr 19, 1995
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A protein having amino acid sequence in SEQ ID NO: 1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID NO: 1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid into E. coli for transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.
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