Method and apparatus for disrupting cells in a fluid suspension by means of a continuous process
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United States of America Patent
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Jul 10, 2007
Grant Date -
Sep 8, 2005
app pub date -
Sep 10, 2004
filing date -
Feb 24, 2004
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Abstract
Method for disrupting S. Cerevisiae yeast cells in an aqueous solution, by means of a continuous process under high pressure (up to 4000 bar), using a homogenizer.The suspension to be processed passes through a homogenizing valve with a 'sharp edge' or 'knife edge' passage head in order to achieve a 100% cell disruption rate with a single passage, at a dynamic pressure equal to or above 2000 bar.The 'sharp edge' or 'knife edge' profile of the passage head (4) is characterized by an inside diameter (9) of 10.9-14 mm and an outside diameter (10) of 11.9 mm-15 mm, and operates at a flow rate of 100-500 liters/hour and a dynamic pressure of more than 2000 bar.
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