Cell-based assays employing voltage and calcium dyes
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United States of America Patent
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Jul 3, 2007
Grant Date -
Oct 6, 2005
app pub date -
Nov 1, 2004
filing date -
Oct 26, 2001
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Abstract
Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.
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