Method for the production of recombinant peptides with a low amount of trisulfides
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United States of America Patent
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Jun 19, 2007
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N/A
app pub date -
Jul 5, 1999
filing date -
Jul 8, 1998
priority date (Note) -
Expired
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Abstract
Methods for the production of recombinant peptides comprise fermenting cells to produce recombinant peptides. A metal salt is added to a concentrate of the fermented cells after the fermentation step, prior to peptide isolation, and the pH of the cell concentrate after the addition of the metal salt is less than or equal to 7, thereby reducing the amount of trifulsides formed in the production of the recombinant peptide, with the proviso that the production does not include conversion of formed trifulside peptide into native peptide form after the addition of the metal salt. In one embodiment, the recombinant peptide is recombinant growth hormone.
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