Medium and method for detecting/identifying microorganisms
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United States of America Patent
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May 15, 2007
Grant Date -
Jan 20, 2005
app pub date -
Nov 16, 2001
filing date -
Nov 17, 2000
priority date (Note) -
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Abstract
A medium for biological analysis by biochemical means involving chromogenic or fluorogenic substrates that react with enzymes (esterases and/or osidases and/or peptidases and/or sulfatases and/or phosphatases) specific for the target strains is that is in a stable, ready-to-use liquid or gel form and contains at least one emulsifying stabilizer of the fatty acid sorbitan ester/fatty acid type and at least one solvent (dimethyl sulfoxide). This medium is obtained by mixing a stock solution of esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase substrates in the solvent, and the emulsifying stabilizer, with some of the constituents of the culture medium.
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