Multiplex analysis using membrane-bound sensitizers
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United States of America Patent
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May 15, 2007
Grant Date -
Feb 2, 2006
app pub date -
Aug 27, 2005
filing date -
Mar 5, 2002
priority date (Note) -
Expired
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Abstract
The present invention is directed to methods and compositions for determining the presence, absence, and/or amounts of one or more membrane-associated analytes in a sample. In accordance with the invention, binding compounds derivatized with releasable molecular tags specifically bind to selected membrane-associated analytes, after which the molecular tags are released upon activation of cleavage moieties, or sensitizers, anchored in the same membrane as the membrane-associated analytes. The released molecular tags are then identified by their distinct separation and detection characteristics.
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